Specific Genomic Alterations in Rat Renal Cell Carcinomas Induced by N-Ethyl-N-hydroxyethylnitrosamine

To characterize genetic alterations occurring in renal tumorigenesis, EHEN-induced renal cell tumors were examined using restriction landmark genomic scanning (RLGS) analysis, an electrophoretic separation technique that detects gene amplifi cations and deletions. Comparison of DNAs from tumor again...

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Bibliographic Details
Published in:Toxicologic pathology 2001-03, Vol.29 (2), p.232-236
Main Authors: Konishi, Noboru, Nakamura, Mitsutoshi, Ishida, Eiwa, Kawada, Yoichi, Nishimine, Masayoshi, Nagai, Hisaki, Emi, Mitsuru
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Biological and medical sciences
Blotting, Southern
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens - toxicity
Carcinoma, Renal Cell - chemically induced
Carcinoma, Renal Cell - genetics
Carcinoma, Renal Cell - pathology
Chemical agents
Diethylnitrosamine - analogs & derivatives
Diethylnitrosamine - toxicity
DNA, Neoplasm - analysis
Genomics
Kidney Neoplasms - chemically induced
Kidney Neoplasms - genetics
Kidney Neoplasms - pathology
Loss of Heterozygosity - genetics
Male
Medical sciences
N-ethyl-N-hydroxyethylnitrosamine
Precancerous Conditions - chemically induced
Precancerous Conditions - genetics
Precancerous Conditions - pathology
Rats
Rats, Wistar
Restriction Mapping - methods
Tumors
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Summary:To characterize genetic alterations occurring in renal tumorigenesis, EHEN-induced renal cell tumors were examined using restriction landmark genomic scanning (RLGS) analysis, an electrophoretic separation technique that detects gene amplifi cations and deletions. Comparison of DNAs from tumor against those from corresponding nontumorous kidney and /or EHEN-treated kidney without development of renal tumors yielded specific alterations in terms of both amplifi ed and reduced DNA spots. Two amplifi ed spots were detected only in renal cell tumors and an additional four spots were frequent in EHEN-treated kidneys. One reduced spot was common to all tumor samples, and another was frequently detected in the tumors analyzed but not in EHEN-treated kidneys. A subset of the altered spots thus appeared to be specific for EHEN-induced renal tumorigenesis.
ISSN:0192-6233
1533-1601
DOI:10.1080/019262301317052503