Genetic analysis of kernel hardness in bread wheat using PCR-based markers

In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred...

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Bibliographic Details
Published in:Theoretical and applied genetics 2001-09, Vol.103 (4), p.601-606
Main Authors: GALANDE, A. A, TIWARI, R, AMMIRAJU, J. S. S, SANTRA, D. K, LAGU, M. D, RAO, V. S, GUPTA, V. S, MISRA, B. K, NAGARAJAN, S, RANJEKAR, P. K
Format: Article
Language:English
Subjects:
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Flour
Fundamental and applied biological sciences. Psychology
Genes
Genetics of eukaryotes. Biological and molecular evolution
Mutation
Proteins
Pteridophyta, spermatophyta
Spectrum analysis
Triticum
Vegetals
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Summary:In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB 208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven linkage groups covering 223.6 cM whereas 11 markers remained unlinked. A multiple-marker model explained the percentage of phenotypic variation for kernel hardness as 20.6%, whereas that for protein content, hectolitre weight and 1,000-kernel weight was 18.8%, 13.5% and 12.1%, respectively. Our results indicate that phenotypic expression of kernel hardness is controlled by many QTLs and is interdependent on various related traits.[PUBLICATION ABSTRACT]
ISSN:0040-5752
1432-2242
DOI:10.1007/pl00002915