The development of sequence-tagged sites (STSs) in Lolium perenne L. : the application of primer sets derived from other genera

Genetic analysis, particularly the development of genetic linkage maps in forage grass species, lags well behind other members of the Poaceae. Comparative mapping within this family has revealed extensive conservation in gene and marker synteny among chromosomes of diverse genera. Recently, the abil...

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Bibliographic Details
Published in:Theoretical and applied genetics 2001-09, Vol.103 (4), p.648-658
Main Authors: TAYLOR, C, MADSEN, K, BORG, S, MØLLER, M. G, BOELT, B, HOLM, P. B
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Barley
Biological and medical sciences
Classical and quantitative genetics. Population genetics. Molecular genetics
Cloning
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genes
Genetics and breeding of economic plants
Genomes
Grasses
Lolium perenne
Molecular genetics
Phalaris coerulescens
Poaceae
Triticum tauchii
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Summary:Genetic analysis, particularly the development of genetic linkage maps in forage grass species, lags well behind other members of the Poaceae. Comparative mapping within this family has revealed extensive conservation in gene and marker synteny among chromosomes of diverse genera. Recently, the ability to transfer mapped STS markers between barley and wheat has been demonstrated. The transfer of mapped STS markers between cereals and forage grasses could provide PCR-based markers for comparative mapping in these species providing they amplify homologous sequences. In this study, primers derived from three barley genes of defined function and a gene from Phalaris coerulescens were used to amplify homologous fragments in Lolium perenne. Primers derived from two barley and two oat cDNA clones were also tested along with eight barley and two Triticum tauchii STS markers. Twenty one primer pairs derived from 18 loci were tested. Eleven primer pairs (52%) amplified homologous sequences in L. perenne from ten (55%) of the loci targetted. Thirteen new STS markers were generated in L. perenne, of which ten have been mapped in barley or rye and amplify homologous sequences in L. perenne.[PUBLICATION ABSTRACT]
ISSN:0040-5752
1432-2242
DOI:10.1007/PL00002920