Involvement of TLR6 in the induction of COX-2, PGE2 and IL-10 in macrophages by lipids from virulent S2P and attenuated R1A Babesia bovis strains

• Published in: Veterinary parasitology 2016-06, Vol.223, p.127-132
• Main Authors: Gimenez, G., Belaunzarán, M.L., Magalhães, K.G., Poncini, C.V., Lammel, E.M., González Cappa, S.M., Bozza, P.T., Isola, E.L.D.
• Format: Article
• Language: English
• Subjects: Animals; Babesia bovis; Babesia bovis - metabolism; Babesia bovis - pathogenicity; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Dinoprostone - genetics; Dinoprostone - metabolism; Interleukin-10 - genetics; Interleukin-10 - metabolism; Lipid Droplets - physiology; Lipid Metabolism - drug effects; Lipids; Macrophages; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - metabolism; Mice; Mice, Knockout; TLR6; Toll-Like Receptor 6 - genetics; Toll-Like Receptor 6 - metabolism; Virulence
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2016.04.033

[Display omitted] •B. bovis lipids (LA and LV) promote lipid body formation in macrophages via TLR6.•TLR6 mediates down-modulation of COX-2/PGE2 and IL-10 induced only by LA.•LA&LV different composition may correlate with polar behaviour of B. bovis strains. Toll like receptors (TLRs) are involved in the modulation of diverse host genes expression through a complex network of signalling events that allow for an appropriate response to a microbial pathogen. In the present work we used TLR6KO mice in order to study the role of TLR6 in the immune discrimination of lipids from two Babesia bovis strains, attenuated R1A (LA) and virulent S2P (LV), and the consequent macrophage activation. We demonstrated that TLR6 is required for lipid body induction in murine peritoneal macrophages by both LA and LV. Interestingly, as regards IL-10 and COX-2/PGE2 pathway induction by LA and LV, we observed differences in the biological effects produced by these lipid extracts. Our results indicate a role of TLR6 in the down-modulation of these immunoregulators only in the case of LA, whereas this receptor was not implicated in pro-inflammatory TNFα, IL-6 and KC release induced by LA. Remarkably, LV did not exert the down-modulatory effect observed for LA, supporting the notion that LA and LV possess different lipid composition that could correlate with the polar pathogenic effect of both B. bovis strains.