A Pycnoporus sanguineus laccase for denim bleaching and its comparison with an enzymatic commercial formulation

A laccase from the basidiomycete Pycnoporus sanguineus strain RVAN5 was evaluated for its ability to decolorize synthetic dyes and denim bleaching. Dye color reduction and denim bleaching were monitored at different dye concentrations and incubation times. Dye decolorization by Pycnoporus sanguineus...

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Bibliographic Details
Published in:Journal of environmental management 2016-07, Vol.177, p.93-100
Main Authors: Iracheta-Cárdenas, María Magdalena, Rocha-Peña, Mario A., Galán-Wong, Luis J., Arévalo-Niño, Katiushka, Tovar-Herrera, Omar Eduardo
Format: Article
Language:English
Subjects:
Barbiturates - chemistry
Basidiomycetes
CIE lab
Color
Coloring Agents - chemistry
Comparative analysis
Denim bleaching
Dye decolorization
Dyes
Enzymes
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Fungi
Laccase - chemistry
Laccase - metabolism
Laccase activity
Pycnoporus
Pycnoporus - chemistry
Pycnoporus - enzymology
Pycnoporus sanguineus
Textiles
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Summary:A laccase from the basidiomycete Pycnoporus sanguineus strain RVAN5 was evaluated for its ability to decolorize synthetic dyes and denim bleaching. Dye color reduction and denim bleaching were monitored at different dye concentrations and incubation times. Dye decolorization by Pycnoporus sanguineus fungal crude extract (FCE) ranged from 80 to 96% within 2–4 h at 25–65 °C. Comparable results were obtained when violuric acid (VA) was added as mediator to the FCE, however, the number of decolorized dyes increased significantly. Dye decolorization rates with VA varied of initial and final optical density (595 nm) values of 2.5–3.0 and 0.2–0.02, respectively. P. sanguineus FCE had no substantial effect on denim bleaching when used alone, notwithstanding, the mixture of FCE with VA (10 mM) showed significant denim color reduction values and considerably higher than those obtained with a bleaching enzyme from a commercial formulation; CIElab values obtained with FCE/VA mixture were of ΔL = 6.4, versus a ΔL 1.4 value obtained with an enzyme from commercial formulation. •Enzyme crude extract decolorizes denim stronger than the commercial formulation.•Violuric acid is required for denim discoloration by the enzyme crude extract.•Enzyme crude extract decolorizes some dyes without mediator.•Putative small laccase protein was found in P. sanguineus.
ISSN:0301-4797
1095-8630
DOI:10.1016/j.jenvman.2016.04.008