Transposable elements and two other molecular markers as typing tools for the genus Paracoccidioides

Studies comparing Paracoccidioides brasiliensis and Paracoccidioides lutzii have shown that these fungi have significant genomic differences that may have implications in the clinical manifestation, diagnosis, and treatment of paracoccidioidomycosis caused by them. Thus, molecular typing methods are...

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Bibliographic Details
Published in:Medical mycology (Oxford) 2015-02, Vol.53 (2), p.165-170
Main Authors: Alves, Fernanda Lourenço, Ribeiro, Mariceli Araújo, Hahn, Rosane Christine, de Melo Teixeira, Marcus, de Camargo, Zoilo Pires, Cisalpino, Patrícia Silva, Marini, Marjorie Mendes
Format: Article
Language:English
Subjects:
DNA Transposable Elements - genetics
Humans
Molecular Typing - methods
Mycological Typing Techniques - methods
Paracoccidioides
Paracoccidioides - classification
Paracoccidioides - genetics
Paracoccidioides - isolation & purification
Paracoccidioides brasiliensis
Paracoccidioidomycosis - microbiology
Polymerase Chain Reaction - methods
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Summary:Studies comparing Paracoccidioides brasiliensis and Paracoccidioides lutzii have shown that these fungi have significant genomic differences that may have implications in the clinical manifestation, diagnosis, and treatment of paracoccidioidomycosis caused by them. Thus, molecular typing methods are required that can distinguish between various species of Paracoccidioides. The aim of this study was to explore the potential use as molecular markers of the transposable elements Trem A-H recently identified and characterized in the genus Paracoccidioides as a means of differentiating the species. We take advantage of the abundance and distribution of these transposons in the Paracoccidioides genomes to develop a simple and highly reproducible polymerase chain reaction (PCR)-based technique. Furthermore we compare the performance of this test with two other molecular markers already in use to identify these fungi.
ISSN:1369-3786
1460-2709
DOI:10.1093/mmy/myu074