Selective fractionation of Sugar Beet Pulp for release of fermentation and chemical feedstocks; optimisation of thermo-chemical pre-treatment

• Published in: Bioresource technology 2016-06, Vol.209, p.259-264
• Main Authors: Hamley-Bennett, C., Lye, G.J., Leak, D.J.
• Format: Article
• Language: English
• Subjects: Arabinose - analysis; Beta vulgaris - chemistry; Bioreactors; Biorefinery; Carbohydrates - analysis; Chemical Fractionation - methods; Chromatography, High Pressure Liquid; Design of experiments; Ethanol - metabolism; Ethanol fermentation; Fermentation; Hydrolysis; Models, Theoretical; Proteins - isolation & purification; Reproducibility of Results; Saccharomyces cerevisiae - metabolism; Solubility; Steam explosion; Sugar Beet Pulp; Temperature
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.02.131

•Valorisation of abundant waste stream.•Optimisation of a steam pre-treatment for (SBP) using statistical design.•Selective fractionation of pectin and cellulose in single step.•No milling of SBP, addition of chemicals or dilution required. The effect of time and pressure on the selective extraction of sugar beet pectin using steam pre-treatment on unprocessed Sugar Beet Pulp was evaluated using a design of experiments approach. This process gave the highest solubilisation of pectin oligomers at a relatively low pressure and longer time (5Bar, 24min), whilst leaving the majority of the cellulose fraction intact. This method of steam pre-treatment fits into the concept of a sugar beet biorefinery as it valorises an existing waste stream without requiring any further physical processing such as milling or dilution with water. The residual cellulose fraction was enriched in cellulose and could be effectively fermented into ethanol by yeast after enzymatic digestion, producing 0.48g ethanol per gram of glucose.