A species-specific activation of Toll-like receptor signaling in bovine and sheep bronchial epithelial cells triggered by Mycobacterial infections

• Published in: Molecular immunology 2016-03, Vol.71, p.23-33
• Main Authors: Ma, Yan, Han, Fei, Liang, Jinping, Yang, Jiali, Shi, Juan, Xue, Jing, Yang, Li, Li, Yong, Luo, Meihui, Wang, Yujiong, Wei, Jun, Liu, Xiaoming
• Format: Article
• Language: English
• Subjects: Airway epithelial cells; Animals; Blotting, Western; Bovine; Bronchi - immunology; Bronchi - metabolism; Bronchi - microbiology; Cattle; Disease Models, Animal; Fluorescent Antibody Technique; Microscopy, Electron; Mycobacterium bovis; Mycobacterium infection; Mycobacterium tuberculosis; Real-Time Polymerase Chain Reaction; Respiratory Mucosa - immunology; Respiratory Mucosa - metabolism; Respiratory Mucosa - microbiology; Sheep; Signal Transduction - immunology; Species Specificity; Species-specific recognition; Toll-like receptor signaling; Toll-Like Receptors - immunology; Toll-Like Receptors - metabolism; Tuberculosis, Pulmonary - immunology; Tuberculosis, Pulmonary - metabolism
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/j.molimm.2016.01.004

•A differentiated bovine bronchial epithelial air–liquid interface model was established.•A species-specific TLR-mediated PAMP recognition in bovine and sheep airway epithelial cells was determined.•Mycobacteria triggered a species-specific TLR signaling in bovine and sheep airway epithelial cells.•Mycobacteria infections induced a species-specific inflammatory response in bovine and sheep airway epithelial cells. Pulmonary tuberculosis caused by a Mycobacterium infection remains a major public health problem in most part of the world, in part owing to the transmission of its pathogens between hosts including human, domestic and wild animals. To date, molecular mechanisms of the pathogenesis of TB are still incompletely understood. In addition to alveolar macrophages, airway epithelial cells have also been recently recognized as main targets for Mycobacteria infections. In an effort to understand the pathogen-host interaction between Mycobacteria and airway epithelial cells in domestic animals, in present study, we investigated the Toll-like receptor (TLR) signaling in bovine and sheep airway epithelial cells in response to an infection of Mycobacterium tuberculosis avirulent H37Ra stain or Mycobacterium bovis BCG vaccine strain, using primary air–liquid interface (ALI) bronchial epithelial culture models. Our results revealed a host and pathogen species-specific TLR-mediated recognition of pathogen-associated molecular patterns (PAMPs), induction and activation of TLR signaling pathways, and substantial induction of inflammatory response in bronchial epithelial cells in response to Mycobacteria infections between these two species. Interestingly, the activation TLR signaling in bovine bronchial epithelial cells induced by Mycobacteria infection was mainly through a myeloid differentiation factor 88 (MyD88)-independent TLR signaling pathway, while both MyD88-dependent and independent TLR signaling cascades could be induced in sheep epithelial cells. Equally noteworthy, a BCG infection was able to induce both MyD88-dependent and independent signaling in sheep and bovine airway epithelial cells, but more robust inflammatory responses were induced in sheep epithelial cells relative to the bovines; whereas an H37Ra infection displayed an ability to mainly trigger a MyD88-independent TLR signaling cascade in these two host species, and induce a more extent expression of inflammatory cytokines in bovine cells in comparison with that in sheep. These data thus pro