HRT dependent performance and bacterial community population of granular hydrogen-producing mixed cultures fed with galactose

•Influence of HRT at a high-rate CSTR fed with galactose has been evaluated.•Peak hydrogen production rate (HPR) of 25.9L/L-d was obtained at 3h HRT.•Biomass concentration dropped from 16 to 3gVSS/L by washout led to the failure.•Increased Clostridium content at optimal HRT (3h) attributed high H2 y...

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Published in:Bioresource technology 2016-04, Vol.206, p.188-194
Main Authors: Kumar, Gopalakrishnan, Sivagurunathan, Periyasamy, Park, Jeong-Hoon, Park, Jong-Hun, Park, Hee-Deung, Yoon, Jeong-Jun, Kim, Sang-Hyoun
Format: Article
Language:English
Subjects:
Bacteria
Bacteria - drug effects
Bacteria - metabolism
Bacteria - ultrastructure
Biofuels
Biomass
Bioreactors - microbiology
Butyrate
Clostridium
Clostridium - drug effects
Clostridium - growth & development
Fermentation - drug effects
Galactose
Galactose - pharmacology
Granular biomass
Hydraulic retention time
Hydrogen - metabolism
Lactate
Phylogeny
Solubility
Time Factors
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Summary:•Influence of HRT at a high-rate CSTR fed with galactose has been evaluated.•Peak hydrogen production rate (HPR) of 25.9L/L-d was obtained at 3h HRT.•Biomass concentration dropped from 16 to 3gVSS/L by washout led to the failure.•Increased Clostridium content at optimal HRT (3h) attributed high H2 yield. The effects of hydraulic retention times (HRTs-6, 3 and 2h) on H2 production, operational stability and bacterial population response in a continuously stirred tank reactor (CSTR) were evaluated using galactose. A peak hydrogen production rate (HPR) of 25.9LH2/L-d was obtained at a 3h HRT with an organic loading rate (OLR) of 120g/L-d, while the maximum hydrogen yield (HY) of 2.21molH2/mol galactose was obtained at a 6h HRT (60ggalactose/L-d). Butyrate was dominant and the lactate concentration increased as HRT decreased, which significantly affected the HY. Biomass concentration (VSS) decreased from 16 to 3g/L at a 2h HRT, leading to failure. A 3h HRT supported the favorable growth of Clostridium species, as indicated by an increase in their populations from 25.4% to 27%, while significantly reducing Bacilli populations from 61.6% to 54.2%, indicating that this was the optimal condition.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.01.104