The effect of oxalic and itaconic acids on threo-Ds-isocitric acid production from rapeseed oil by Yarrowia lipolytica

• Published in: Bioresource technology 2016-04, Vol.206, p.128-133
• Main Authors: Kamzolova, Svetlana V., Allayarov, Ramil K., Lunina, Julia N., Morgunov, Igor G.
• Format: Article
• Language: English
• Subjects: Citric Acid - metabolism; Enzyme Inhibitors - metabolism; Enzyme Inhibitors - pharmacology; Fatty Acids, Monounsaturated; Industrial Microbiology - methods; Isocitrate lyase; Isocitrate Lyase - antagonists & inhibitors; Isocitrate Lyase - metabolism; Isocitrates - metabolism; Itaconic acid; Oxalic acid; Oxalic Acid - metabolism; Oxalic Acid - pharmacology; Plant Oils - metabolism; Rapeseed Oil; Succinates - metabolism; Succinates - pharmacology; Threo-Ds-isocitric acid; Yarrowia - drug effects; Yarrowia - metabolism; Yarrowia lipolytica; Yarrowia lipolytica yeast
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.01.092

•Threo-Ds-isocitric acid for food industry and sport medicine.•The microbiological synthesis of isocitric acid from rapeseed oil by yeast.•Oxalic and itaconic acids are the strong inhibitors of isocitrate lyase of Y. lipolytica.•Inhibition of isocitrate lyase shifts metabolism toward the isocitrate production. The effect of oxalic and itaconic acids, the inhibitors of the isocitrate lyase, on the production of isocitric acid by the wild strain Yarrowia lipolytica VKM Y-2373 grown in the medium containing rapeseed oil was studied. In the presence of oxalic and itaconic acids, strain Y. lipolytica accumulated in the medium isocitric acid (70.0 and 82.7g/L, respectively) and citric acid (23.0 and 18.4g/L, respectively). In control experiment, when the inhibitors were not added to the medium, the strain accumulated isocitric and citric acids at concentrations of 62.0 and 28.0g/L, respectively. Thus, the use of the oxalic and itaconic acids as additives to the medium is a simple and convenient method of isocitric acid production with a minimum content of citric acid.