Role of PHLPP1 in inflammation response: Its loss contributes to gliomas development and progression

• Published in: International immunopharmacology 2016-05, Vol.34, p.229-234
• Main Authors: Teng, Da-Cai, Sun, Juan, An, Yi-Qiang, Hu, Zhong-Hao, Liu, Peng, Ma, Yong-Chao, Han, Bing, Shi, Yang
• Format: Article
• Language: English
• Subjects: Brain Neoplasms - immunology; Brain Neoplasms - pathology; Carcinogenesis; Cell Line; Cytokines - genetics; Cytokines - metabolism; Female; Gene Expression Regulation, Neoplastic; Glioma; Glioma - immunology; Glioma - pathology; Humans; Inflammation; Inflammation Mediators - metabolism; Male; Neoplasm Staging; Nuclear Proteins - genetics; Nuclear Proteins - immunology; PHLPP1; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - immunology; RNA, Small Interfering - genetics; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Tumor suppressors
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2016.02.034

PH domain leucine-rich repeats protein phosphatase 1(PHLPP1) belongs to a novel family of Ser/Thr protein phosphatases: PHLPP serves as tumor suppressor in several cancers. However, little knowledge about the expression of PHLPP1 in human glioma tumor tissue and its role in inflammation response in glioma cells was known. Glioma samples were obtained from a total of 37 patients including 16 males and 21 females with surgical removal of the brain tumor. PHLPP1 protein and inflammatory cytokines were measured by Western blot analysis and immunohistochemistry while mRNA was determined by RT-PCR. The levels of inflammatory cytokines including TNF-α, IL-17, IL-1β in U251 glioma cells were evaluated by siRNA PHLPP1 and PHLPP1 addition. The loss of PHLPP1 expression occurs at high frequency in human gliomas. The highest mean values of PHLPP1 mRNA and protein were found in non-glioma brain tissues whereas the lowest mean values were found in those in glioblastoma with an increase of TNF-α, IL-17, IL-1β (p