Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP

PCR-RFLP analyses of mpi gene fragments digested with VpaK11BI. [Display omitted] •A PCR-RFLP was established to differentiate Leishmania species in Huanuco, Peru.•The technique was applied to 267 DNA samples from patients with cutaneous leishmaniasis.•All the PCR-amplified samples were successfully...

Full description

Saved in:
Bibliographic Details
Published in:Acta tropica 2016-06, Vol.158, p.83-87
Main Authors: Koarashi, Yu, Cáceres, Abraham G., Saca, Florencia Margarita Zúniga, Flores, Elsa Elvira Palacios, Trujillo, Adela Celis, Alvares, José Luis Abanto, Yoshimatsu, Kumiko, Arikawa, Jiro, Katakura, Ken, Hashiguchi, Yoshihisa, Kato, Hirotomo
Format: Article
Language:English
Subjects:
Azure Stains
Humans
Leishmania
Leishmania - classification
Leishmania - genetics
Leishmaniasis, Cutaneous - epidemiology
Leishmaniasis, Cutaneous - genetics
Mannose phosphate isomerase
PCR-RFLP
Peru
Peru - epidemiology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Viannia braziliensis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:PCR-RFLP analyses of mpi gene fragments digested with VpaK11BI. [Display omitted] •A PCR-RFLP was established to differentiate Leishmania species in Huanuco, Peru.•The technique was applied to 267 DNA samples from patients with cutaneous leishmaniasis.•All the PCR-amplified samples were successfully identified at the species level.•The technique will be a useful tool for epidemiological study of leishmaniasis. A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2016.02.024