High calcium diet improves the liver oxidative stress and microsteatosis in adult obese rats that were overfed during lactation

• Published in: Food and chemical toxicology 2016-06, Vol.92, p.245-255
• Main Authors: Conceição, E.P.S., Moura, E.G., Soares, P.N., Ai, X.X., Figueiredo, M.S., Oliveira, E., Lisboa, P.C.
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Antioxidants - metabolism; Blotting, Western; Body Weight; Calcium - administration & dosage; Calcium - pharmacology; Diet; Fatty Liver - metabolism; Fatty Liver - pathology; Fatty Liver - prevention & control; Female; High calcium diet; Insulin Resistance; Lactation; Liver Diseases - metabolism; Liver Diseases - pathology; Liver Diseases - prevention & control; Liver dysfunction; Male; Obesity - physiopathology; Overnutrition; Overnutrition - physiopathology; Oxidative stress; Oxidative Stress - drug effects; Rats; Rats, Wistar
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2016.04.015

Obesity is related to diabetes, higher oxidative stress and nonalcoholic fatty liver disease, and dietetic therapies, for instance calcium-rich diet, can improve these dysfunctions. Rats raised in small litters (SL) had increased fat depots and insulin resistance at adulthood associated with higher liver oxidative stress and microsteatosis. Thus, we evaluated if dietary calcium can improve these changes. In PN3, litter size was adjusted to 3 pups (SL group) to induce overfeeding, while controls had 10 pups until weaning. At PN120, SL group was randomly divided into: rats fed with standard chow or fed with calcium supplementation (SL–Ca group, 10 g/kg chow) for 60 days. At PN180, dietary calcium normalized food consumption, visceral fat, plasma aspartate aminotransferase (AST) and glycaemia. Concerning oxidative balance, calcium restored both higher hepatic lipid peroxidation and protein carbonylation as well as higher plasma lipid peroxidation. Higher fatty acid synthase (FAS) content, steatosis and lower protein kinase B (Akt) in SL group were normalized by dietary calcium and SL–Ca rats had lower hepatic cholesterol. Thus, calcium supplementation improved the insulin sensitivity, redox balance and steatosis in the liver. Therefore, dietary calcium can be a promising therapy for liver disease in the metabolic syndrome. •Overfed rats have higher fat depot and insulin resistance at adulthood associated with liver oxidative stress and steatosis.•Calcium supplementation was used to correct the metabolic syndrome due to its antioxidant and anti-lipogenic properties.•High calcium intake improved the redox balance in obese rats and normalized the liver steatosis.