Transcranial direct current stimulation improves short-term memory in an animal model of attention-deficit/hyperactivity disorder

• Published in: European neuropsychopharmacology 2016-02, Vol.26 (2), p.368-377
• Main Authors: Leffa, Douglas Teixeira, de Souza, Andressa, Scarabelot, Vanessa Leal, Medeiros, Liciane Fernandes, de Oliveira, Carla, Grevet, Eugenio Horacio, Caumo, Wolnei, de Souza, Diogo Onofre, Rohde, Luis Augusto Paim, Torres, Iraci L.S
• Format: Article
• Language: English
• Subjects: ADHD; Analysis of Variance; Animals; Attention Deficit Disorder with Hyperactivity - complications; Attention Deficit Disorder with Hyperactivity - pathology; Brain - metabolism; Brain-Derived Neurotrophic Factor - metabolism; Disease Models, Animal; Dopamine; Dopamine - metabolism; Internal Medicine; Locomotion - physiology; Male; Memory; Memory Disorders - etiology; Memory Disorders - therapy; Memory, Short-Term - physiology; Psychiatry; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Recognition (Psychology) - physiology; SHR; Statistics as Topic; tDCS; Transcranial Direct Current Stimulation
• ISSN: 0924-977X; 1873-7862
• DOI: 10.1016/j.euroneuro.2015.11.012

Abstract Attention deficit hyperactivity disorder (ADHD) is characterized by impairing levels of hyperactivity, impulsivity and inattention. However, different meta-analyses have reported disruptions in short and long-term memory in ADHD patients. Previous studies indicate that mnemonic dysfunctions might be the result of deficits in attentional circuits, probably due to ineffective dopaminergic modulation of hippocampal synaptic plasticity. In this study we aimed to evaluate the potential therapeutic effects of a neuromodulatory technique, transcranial direct current stimulation (tDCS), in short-term memory (STM) deficits presented by the spontaneous hypertensive rats (SHR), the most widely used animal model of ADHD. Adult male SHR and Wistar Kyoto rats (WKY) were subjected to a constant electrical current of 0.5 mA intensity applied on the frontal cortex for 20 min/day during 8 days. STM was evaluated with an object recognition test conducted in an open field. Exploration time and locomotion were recorded, and brain regions were dissected to determine dopamine and BDNF levels. SHR spent less time exploring the new object when compared to WKY, and tDCS improved object recognition deficits in SHR without affecting WKY performance. Locomotor activity was higher in SHR and it was not affected by tDCS. After stimulation, dopamine levels were increased in the hippocampus and striatum of both strains, while BDNF levels were increased only in the striatum of WKY. These findings suggest that tDCS on the frontal cortex might be able to improve STM deficits present in SHR, which is potentially related to dopaminergic neurotransmission in the hippocampus and striatum of those animals.