The use of RNAi to analyze gene function in spirotrichous ciliates

The use of small double-stranded RNA to inhibit expression of genes has proven to be a powerful tool to analyze the biological function of genes in various eukaryotic organisms. We have established the RNAi technology in three genera of spirotrichous ciliates, Euplotes, Oxytricha and Stylonychia. He...

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Bibliographic Details
Published in:European journal of protistology 2003-12, Vol.39 (4), p.449-454
Main Authors: Paschka, Adrian G., Jönsson, Franziska, Maier, Verena, Möllenbeck, Matthias, Paeschke, Katrin, Postberg, Jan, Rupprecht, Sina, Lipps, Hans J.
Format: Article
Language:English
Subjects:
Euplotes
Oxytricha
Stylonychia
telomere-binding protein
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Summary:The use of small double-stranded RNA to inhibit expression of genes has proven to be a powerful tool to analyze the biological function of genes in various eukaryotic organisms. We have established the RNAi technology in three genera of spirotrichous ciliates, Euplotes, Oxytricha and Stylonychia. Here we describe this method to silence gene expression in these three genera. Expression of the following genes was inhibited: the catalytic subunit of telomerase (TERT) and the telomerase-associated protein p43 in Euplotes and the alpha -subunit of the telomere-binding protein ( alpha TeBP) of Stylonychia and Oxytricha. In addition, we report how the biological function of a developmentally regulated gene can be elucidated by this technique in Stylonychia.
ISSN:0932-4739
1618-0429
DOI:10.1078/0932-4739-00019