DNA damage and repair in Arabidopsis thaliana as measured by the comet assay after treatment with different classes of genotoxins

The three protocols of the comet assay A/N, A/A and N/N were for the first time applied to the plant species Arabidopsis thaliana. The purpose of the experiments was to establish conditions for genotoxic exposure causing DNA damage in Arabidopsis nuclei. This is required for comprehensive gene expre...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2001-06, Vol.493 (1), p.87-93
Main Authors: Menke, Merten, Chen, I-Peng, Angelis, Karel J, Schubert, Ingo
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Biological and medical sciences
Bleomycin
Comet assay
Fundamental and applied biological sciences. Psychology
Maleic hydrazide
methyl methanesulfonate
Mitomycin C
Molecular and cellular biology
Molecular genetics
Monofunctioal alkylating agents
Mutagenesis. Repair
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Summary:The three protocols of the comet assay A/N, A/A and N/N were for the first time applied to the plant species Arabidopsis thaliana. The purpose of the experiments was to establish conditions for genotoxic exposure causing DNA damage in Arabidopsis nuclei. This is required for comprehensive gene expression profiling with the intention to screen for genes involved in response of Arabidopsis cells to genotoxic stress. Five chemicals belonging to different classes of mutagens (the monofunctional alkylating agents N-methyl- N-nitrosourea and methyl methanesulfonate, the polyfunctional alkylating agent mitomycin C, the radiomimetic bleomycin and the herbicide maleic hydrazide) were tested. Except for maleic hydrazide, dose-dependent increases in DNA damage were found using the A/N comet assay protocol. While a rapid repair of bleomycin-mediated SSBs and DSBs was found, no significant reduction of DNA migration was observed up to 48 h after treatment with the monofunctional alkylating agents.
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(01)00165-6