Mass Spectrometry for Paper-Based Immunoassays: Toward On‑Demand Diagnosis

Current analytical methods, either point-of-care or centralized detection, are not able to meet recent demands of patient-friendly testing and increased reliability of results. Here, we describe a two-point separation on-demand diagnostic strategy based on a paper-based mass spectrometry immuno­assa...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2016-05, Vol.138 (20), p.6356-6359
Main Authors: Chen, Suming, Wan, Qiongqiong, Badu-Tawiah, Abraham K
Format: Article
Language:English
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Summary:Current analytical methods, either point-of-care or centralized detection, are not able to meet recent demands of patient-friendly testing and increased reliability of results. Here, we describe a two-point separation on-demand diagnostic strategy based on a paper-based mass spectrometry immuno­assay platform that adopts stable and cleavable ionic probes as mass reporter; these probes make possible sensitive, interruptible, storable, and restorable on-demand detection. In addition, a new touch paper spray method was developed for on-chip, sensitive, and cost-effective analyte detection. This concept is successfully demonstrated via (i) the detection of Plasmodium falciparum histidine-rich protein 2 antigen and (ii) multiplexed and simultaneous detection of cancer antigen 125 and carcinoembryonic antigen.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.6b02232