TFIIIB subunit locations on U6 gene promoter DNA mapped by site‐specific protein–DNA photo‐cross‐linking

RNA polymerase III‐transcribed U6 snRNA genes have gene‐external promoters that contain TATA boxes. U6 TATA sequences are bound by TFIIIB that in Drosophila contains the three subunits TBP, Brf1, and Bdp1. The overall structure of TFIIIB is still not well understood. We have therefore studied the mo...

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Bibliographic Details
Published in:FEBS letters 2016-05, Vol.590 (10), p.1488-1497
Main Authors: Kang, Jin Joo, Kang, Yoon Soon, Stumph, William E.
Format: Article
Language:English
Subjects:
Animals
Bdp1
Binding Sites
Brf1
Cell Line
Cross-Linking Reagents
Drosophila - metabolism
Drosophila Proteins - chemistry
Drosophila Proteins - metabolism
Models, Molecular
Promoter Regions, Genetic
Protein Binding
Protein–DNA photo‐cross‐linking
RNA polymerase III transcription
RNA, Small Nuclear - chemistry
RNA, Small Nuclear - metabolism
TATA Box
TATA box‐binding protein
TATA-Box Binding Protein - chemistry
TATA-Box Binding Protein - metabolism
TFIIIB
Transcription Factor TFIIIB - chemistry
Transcription Factor TFIIIB - metabolism
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Summary:RNA polymerase III‐transcribed U6 snRNA genes have gene‐external promoters that contain TATA boxes. U6 TATA sequences are bound by TFIIIB that in Drosophila contains the three subunits TBP, Brf1, and Bdp1. The overall structure of TFIIIB is still not well understood. We have therefore studied the mode of TFIIIB binding to DNA by site‐specific protein–DNA photo‐cross‐linking. The results indicate that a portion of Brf1 is sandwiched between Bdp1 and TBP upstream of the TATA box. Furthermore, Bdp1 traverses the DNA under the N‐terminal stirrup of TBP to interact with the DNA (and very likely Brf1) downstream of the TATA sequence.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12185