Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation

Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, inclu...

Full description

Saved in:
Bibliographic Details
Published in:Stem cell reviews and reports 2016-06, Vol.12 (3), p.298-304
Main Authors: Xiong, Yujing, Wang, Enyin, Huang, Yan, Guo, Xiaoyi, Yu, Yiping, Du, Qingyun, Ding, Xiaoyan, Sun, Yingpu
Format: Article
Language:English
Subjects:
Adipocytes - metabolism
Adipocytes - physiology
Adipogenesis - physiology
Animals
Cell Differentiation - physiology
Cell Line
Cell Proliferation - physiology
DNA Methylation - physiology
Epigenesis, Genetic - physiology
Histone Demethylases - metabolism
Histones - metabolism
Human Embryonic Stem Cells - metabolism
Human Embryonic Stem Cells - physiology
Humans
Mice
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c503t-dfd05dcc2937f4d6d010df8e34fb66a22eeb8a45095e5c41790f3a26bdfd1943
cites
container_end_page 304
container_issue 3
container_start_page 298
container_title Stem cell reviews and reports
container_volume 12
creator Xiong, Yujing
Wang, Enyin
Huang, Yan
Guo, Xiaoyi
Yu, Yiping
Du, Qingyun
Ding, Xiaoyan
Sun, Yingpu
description Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs.
doi_str_mv 10.1007/s12015-016-9650-z
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1791751751</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1791751751</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-dfd05dcc2937f4d6d010df8e34fb66a22eeb8a45095e5c41790f3a26bdfd1943</originalsourceid><addsrcrecordid>eNpdkD1PwzAQhi0EAgT9ASzIEgsMpmc7dpKxaoFWbQVSu1f5OBOjJg5xMpSRX06qUgakk-6G53l1egm54fDIAcKh5wK4YsA1i7UC9nVCLoUWMZMiDE__bh1fkIH3HwAgJAS9ck4uRAgqjnR0Sb5nVWFT21pXUWfoYudthWxVY2aNzegES2yL3TbxyDi9X6wmfDifLPnogb41rnQtetoWSEe5rd07VnvFGoMNVq1NjqnF02rs6bpoXPde0KmcB3R5iN0T1-TMJFuPg999RdbPT-vxlC1eX2bj0YJlCmTLcpODyrNMxDI0Qa5z4JCbCGVgUq0TIRDTKAkUxApVFvAwBiMTodNe5HEgr8j9IbZu3GeHvt2U1me43SYVus5veoGHaj89evcP_XBdU_XP7SmI-uaU7qnbX6pLS8w3dWPLpNltjuXKH-y1e9A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1790886856</pqid></control><display><type>article</type><title>Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation</title><source>Springer Nature</source><creator>Xiong, Yujing ; Wang, Enyin ; Huang, Yan ; Guo, Xiaoyi ; Yu, Yiping ; Du, Qingyun ; Ding, Xiaoyan ; Sun, Yingpu</creator><creatorcontrib>Xiong, Yujing ; Wang, Enyin ; Huang, Yan ; Guo, Xiaoyi ; Yu, Yiping ; Du, Qingyun ; Ding, Xiaoyan ; Sun, Yingpu</creatorcontrib><description>Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs.</description><identifier>ISSN: 2629-3269</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-016-9650-z</identifier><identifier>PMID: 27059868</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Adipocytes - metabolism ; Adipocytes - physiology ; Adipogenesis - physiology ; Animals ; Cell Differentiation - physiology ; Cell Line ; Cell Proliferation - physiology ; DNA Methylation - physiology ; Epigenesis, Genetic - physiology ; Histone Demethylases - metabolism ; Histones - metabolism ; Human Embryonic Stem Cells - metabolism ; Human Embryonic Stem Cells - physiology ; Humans ; Mice</subject><ispartof>Stem cell reviews and reports, 2016-06, Vol.12 (3), p.298-304</ispartof><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-dfd05dcc2937f4d6d010df8e34fb66a22eeb8a45095e5c41790f3a26bdfd1943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27059868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiong, Yujing</creatorcontrib><creatorcontrib>Wang, Enyin</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Guo, Xiaoyi</creatorcontrib><creatorcontrib>Yu, Yiping</creatorcontrib><creatorcontrib>Du, Qingyun</creatorcontrib><creatorcontrib>Ding, Xiaoyan</creatorcontrib><creatorcontrib>Sun, Yingpu</creatorcontrib><title>Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation</title><title>Stem cell reviews and reports</title><addtitle>Stem Cell Rev Rep</addtitle><description>Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs.</description><subject>Adipocytes - metabolism</subject><subject>Adipocytes - physiology</subject><subject>Adipogenesis - physiology</subject><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell Proliferation - physiology</subject><subject>DNA Methylation - physiology</subject><subject>Epigenesis, Genetic - physiology</subject><subject>Histone Demethylases - metabolism</subject><subject>Histones - metabolism</subject><subject>Human Embryonic Stem Cells - metabolism</subject><subject>Human Embryonic Stem Cells - physiology</subject><subject>Humans</subject><subject>Mice</subject><issn>2629-3269</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNpdkD1PwzAQhi0EAgT9ASzIEgsMpmc7dpKxaoFWbQVSu1f5OBOjJg5xMpSRX06qUgakk-6G53l1egm54fDIAcKh5wK4YsA1i7UC9nVCLoUWMZMiDE__bh1fkIH3HwAgJAS9ck4uRAgqjnR0Sb5nVWFT21pXUWfoYudthWxVY2aNzegES2yL3TbxyDi9X6wmfDifLPnogb41rnQtetoWSEe5rd07VnvFGoMNVq1NjqnF02rs6bpoXPde0KmcB3R5iN0T1-TMJFuPg999RdbPT-vxlC1eX2bj0YJlCmTLcpODyrNMxDI0Qa5z4JCbCGVgUq0TIRDTKAkUxApVFvAwBiMTodNe5HEgr8j9IbZu3GeHvt2U1me43SYVus5veoGHaj89evcP_XBdU_XP7SmI-uaU7qnbX6pLS8w3dWPLpNltjuXKH-y1e9A</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Xiong, Yujing</creator><creator>Wang, Enyin</creator><creator>Huang, Yan</creator><creator>Guo, Xiaoyi</creator><creator>Yu, Yiping</creator><creator>Du, Qingyun</creator><creator>Ding, Xiaoyan</creator><creator>Sun, Yingpu</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160601</creationdate><title>Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation</title><author>Xiong, Yujing ; Wang, Enyin ; Huang, Yan ; Guo, Xiaoyi ; Yu, Yiping ; Du, Qingyun ; Ding, Xiaoyan ; Sun, Yingpu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-dfd05dcc2937f4d6d010df8e34fb66a22eeb8a45095e5c41790f3a26bdfd1943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adipocytes - metabolism</topic><topic>Adipocytes - physiology</topic><topic>Adipogenesis - physiology</topic><topic>Animals</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell Proliferation - physiology</topic><topic>DNA Methylation - physiology</topic><topic>Epigenesis, Genetic - physiology</topic><topic>Histone Demethylases - metabolism</topic><topic>Histones - metabolism</topic><topic>Human Embryonic Stem Cells - metabolism</topic><topic>Human Embryonic Stem Cells - physiology</topic><topic>Humans</topic><topic>Mice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiong, Yujing</creatorcontrib><creatorcontrib>Wang, Enyin</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Guo, Xiaoyi</creatorcontrib><creatorcontrib>Yu, Yiping</creatorcontrib><creatorcontrib>Du, Qingyun</creatorcontrib><creatorcontrib>Ding, Xiaoyan</creatorcontrib><creatorcontrib>Sun, Yingpu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cell reviews and reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiong, Yujing</au><au>Wang, Enyin</au><au>Huang, Yan</au><au>Guo, Xiaoyi</au><au>Yu, Yiping</au><au>Du, Qingyun</au><au>Ding, Xiaoyan</au><au>Sun, Yingpu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation</atitle><jtitle>Stem cell reviews and reports</jtitle><addtitle>Stem Cell Rev Rep</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>12</volume><issue>3</issue><spage>298</spage><epage>304</epage><pages>298-304</pages><issn>2629-3269</issn><eissn>2629-3277</eissn><abstract>Given their totipotency, human embryonic stem cells (hESCs) can differentiate into all types of cells, including adipocytes, and provide an excellent research model for studying diseases associated with the metabolism of adipocytes, such as obesity and diabetes mellitus. Epigenetic regulation, including DNA methylation and histone modification, plays an essential role in the development and differentiation of hESCs. Lysine-specific demethylase 1 (LSD1), a well-characterized histone-modifying enzyme, demethylates dimethylated histone H3 lysine 4 (H3K4) through a flavin adenine dinucleotide (FAD)-dependent oxidative reaction. LSD1 affects the growth and differentiation of human and mouse ES cells, and the deletion of this gene in mice leads to embryonic lethality. Here, we investigated the functional role of LSD1 during the adipogenic differentiation of hESCs involving the demethylation of H3K4. We also found that treating hESCs with the LSD1 inhibitor CBB1007 promotes the adipogenic differentiation of hESCs.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>27059868</pmid><doi>10.1007/s12015-016-9650-z</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2629-3269
ispartof Stem cell reviews and reports, 2016-06, Vol.12 (3), p.298-304
issn 2629-3269
2629-3277
language eng
recordid cdi_proquest_miscellaneous_1791751751
source Springer Nature
subjects Adipocytes - metabolism
Adipocytes - physiology
Adipogenesis - physiology
Animals
Cell Differentiation - physiology
Cell Line
Cell Proliferation - physiology
DNA Methylation - physiology
Epigenesis, Genetic - physiology
Histone Demethylases - metabolism
Histones - metabolism
Human Embryonic Stem Cells - metabolism
Human Embryonic Stem Cells - physiology
Humans
Mice
title Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T09%3A06%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibition%20of%20Lysine-Specific%20Demethylase-1%20(LSD1/KDM1A)%20Promotes%20the%20Adipogenic%20Differentiation%20of%20hESCs%20Through%20H3K4%20Methylation&rft.jtitle=Stem%20cell%20reviews%20and%20reports&rft.au=Xiong,%20Yujing&rft.date=2016-06-01&rft.volume=12&rft.issue=3&rft.spage=298&rft.epage=304&rft.pages=298-304&rft.issn=2629-3269&rft.eissn=2629-3277&rft_id=info:doi/10.1007/s12015-016-9650-z&rft_dat=%3Cproquest_pubme%3E1791751751%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c503t-dfd05dcc2937f4d6d010df8e34fb66a22eeb8a45095e5c41790f3a26bdfd1943%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1790886856&rft_id=info:pmid/27059868&rfr_iscdi=true