Immunogenicity of the histidine-to-tyrosine staphylococcal enterotoxin B mutant protein in C3H/HeJ mice

Staphylococcal enterotoxin B (SEB) is a common cause of food poisoning and toxic shock. A safe and effective vaccine is needed to protect against the superantigenic effects of this toxin. We previously constructed and produced an apparently nontoxic SEB mutant having four histidine-to-tyrosine subst...

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Bibliographic Details
Published in:Toxicon (Oxford) 2004-03, Vol.43 (4), p.433-438
Main Authors: Savransky, Vladimir, Pinelis, Dmitriy, Korolev, Sergey, Ionin, Boris, Fegeding, Konstantin
Format: Article
Language:English
Subjects:
Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
Dactinomycin
Enterotoxins - genetics
Enterotoxins - immunology
Enterotoxins - toxicity
Enzyme-Linked Immunosorbent Assay
Female
Immune Tolerance
Immunity
Immunoglobulin G - biosynthesis
Immunoglobulin G - immunology
Medical sciences
Mice
Mice, Inbred C3H
Mutation - genetics
Staphylococcal Vaccines - immunology
Staphylococcus
Staphylococcus aureus - immunology
Superantigen
Superantigens - genetics
Superantigens - immunology
Toxic shock
Toxicology
Tyrosine
Vaccine
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Summary:Staphylococcal enterotoxin B (SEB) is a common cause of food poisoning and toxic shock. A safe and effective vaccine is needed to protect against the superantigenic effects of this toxin. We previously constructed and produced an apparently nontoxic SEB mutant having four histidine-to-tyrosine substitutions in positions 12, 32, 105, and 121. In the present study, we found that this H1.2.3.4 SEB mutant had low toxicity, was able to induce high levels of specific IgG antibodies, and protected mice in both the actinomycin D-primed and intranasal SEB intoxication model systems, despite the absence of detectable specific IgM and IgA antibodies. We propose further development of the H1.2.3.4 recombinant protein as a potential anti-SEB vaccine candidate.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2004.02.013