Mitotic Cycles in Oxygen Uptake and Carbon Dioxide Output in the Cleaving Frog Egg

Rates of oxygen uptake and of carbon dioxide output of single cleaving frog eggs were measured at short intervals. A Cartesian diver modified for the purpose was used in conjunction with a sensitive manometer. Carbon dioxide outputs were measured as the difference between results obtained with and w...

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Bibliographic Details
Published in:The Biological bulletin (Lancaster) 1972-12, Vol.143 (3), p.699-706
Main Authors: Zeuthen, Erik, Hamburger, Kirsten
Format: Article
Language:English
Subjects:
Amplitude
Animals
Anura
Carbon dioxide
Carbon Dioxide - biosynthesis
Diving
Eggs
Female
Fluids
Freshwater
Frogs
Mitosis
Ovum - metabolism
Oxygen
Oxygen Consumption
Ranidae - embryology
Respiration
Vapor phases
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Summary:Rates of oxygen uptake and of carbon dioxide output of single cleaving frog eggs were measured at short intervals. A Cartesian diver modified for the purpose was used in conjunction with a sensitive manometer. Carbon dioxide outputs were measured as the difference between results obtained with and without absorption of CO2 in the system. For both measured parameters reproducible variations were found to accompany the mitotic cycle. Oxygen uptake occurred at minimal rate shortly before the beginning of each new division (cleavage furrow) and at maximal rate halfway between two maxima. Rates of carbon dioxide production varied in an inverse manner and at the same time were more pronounced. Consequently, RQ cycled regularly around a mean (0.6-0.7) and with an amplitude of about 0.1-0.15 units. RQ maxima were at 10-15 min before, minima at 10-20 min after the beginning of furrow formation.
ISSN:0006-3185
1939-8697
DOI:10.2307/1540192