Mycosporine-Like Amino Acid Intercalibration Effort Using Replicate Samples

The measurement of mycosporine‐like amino acids (MAAs) has several inherent problems, including the lack of commercial standards for identification and quantification. This symposium provided the opportunity to coordinate analyses with six laboratories actively involved in MAA research. Two samples...

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Bibliographic Details
Published in:Journal of phycology 2002-06, Vol.38 (s1), p.41-41
Main Authors: Zimba, P. V., Boue, S.
Format: Article
Language:English
Subjects:
Brackish
Cyanophyta
Freshwater
Marine
Microcystis aeruginosa
Mycosporine-like amino acids
Porphyra
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Summary:The measurement of mycosporine‐like amino acids (MAAs) has several inherent problems, including the lack of commercial standards for identification and quantification. This symposium provided the opportunity to coordinate analyses with six laboratories actively involved in MAA research. Two samples were provided to each laboratory and included freeze‐dried nori (Porphyra sp.) and a freeze‐dried (filtered) sample of the cyanobacteria Microcystis aeruginosa Kützing. Each laboratory provided extraction methodologies, chromatograms of the identified peaks, as well as estimates of the concentration of each analyte. All laboratories were able to identify major chromatographic components of the samples (Porphyra: shinorine, porphyra, mycosporine 2‐glycine, asterina, palythine; Microcystis: shinorine, porphyra). Sequential cold (4 °C for 18 h) and hot (45 °C for 2 h) extractions of the same sample resulted in differing analyte recovery. Several currently unidentified compounds were observed in freshwater samples.
ISSN:0022-3646
1529-8817
DOI:10.1046/j.1529-8817.38.s1.11.x