Comparison of vitrified outcomes between human early blastocysts and expanded blastocysts

We compared the vitrified outcomes between early and expanded blastocysts with or without laser drilling. The grade III embryos from the patients undergoing in vitro fertilization-embryo transfer (IVF-ET) in our reproductive center from September 2009 to February 2015 were incubated into early blast...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2016-05, Vol.52 (5), p.522-529
Main Authors: Song, Wen-yan, Wang, Xue-gai, Jin, Hai-xia, Yao, Gui-dong, Zhang, Xiang-yang, Shi, Sen-lin, Yang, Hong-yi, Peng, Zhao-feng, Sun, Ying-pu
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Apoptosis
Biomedical and Life Sciences
BIOTECHNOLOGY
Blastocyst
Blastocyst - cytology
Blastocyst - physiology
Cell Biology
Cell Culture
Control groups
Cryopreservation - methods
Developmental Biology
Drilling
Embryonic Development
Embryos
Frost damage
Hatching
Humans
In Situ Nick-End Labeling
Lasers
Life Sciences
Stem Cells
Thawing
Vitrification
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Summary:We compared the vitrified outcomes between early and expanded blastocysts with or without laser drilling. The grade III embryos from the patients undergoing in vitro fertilization-embryo transfer (IVF-ET) in our reproductive center from September 2009 to February 2015 were incubated into early blastocysts and expanded blastocysts. The early blastocysts and expanded blastocysts were, respectively, divided into laser group (vitrification after laser drilling), non-laser group (direct vitrification), and control group (fresh non-vitrified blastocysts). After thawing, the blastular anabiosis rate, expansion rate, hatching rate, and apoptosis were observed in each group and then were compared amongst groups. This study indicated that the blastular expansion rate (all P < 0.01) and hatching rate (all P < 0.01) were significantly lower, but the blastular apoptosis (all P < 0.05) was significantly higher in both laser and non-laser groups than in the control group in the early blastocysts. In the expanded blastocysts, the blastular anabiosis rate was significantly higher in the laser group than in the non-laser group (P < 0.0), and the blastular expansion rate was significantly higher, but the blastular apoptosis was significantly lower in both laser group and control group than in the non-laser group (all P < 0.05). The blastular expansion rate (all P < 0.01) and hatching rate (all P < 0.01) were significantly higher, but the blastular apoptosis (all P < 0.05) was significantly lower in the expanded laser group than in both early laser and early non-laser groups. We conclude that vitrification for laser-drilling expanded blastocysts can achieve the best outcomes.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-016-0009-1