Connective tissue growth factor is activated by gastrin and involved in gastrin-induced migration and invasion

Connective tissue growth factor (CTGF) has been reported in gastric adenocarcinoma and in carcinoid tumors. The aim of this study was to explore a possible link between CTGF and gastrin in gastric epithelial cells and to study the role of CTGF in gastrin induced migration and invasion of AGS-GR cell...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2016-06, Vol.475 (1), p.119-124
Main Authors: Bhandari, Sabin, Bakke, Ingunn, Kumar, J., Beisvag, Vidar, Sandvik, Arne K., Thommesen, Liv, Varro, Andrea, Nørsett, Kristin G.
Format: Article
Language:English
Subjects:
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Animals
Cell Movement
Connective tissue growth factor
Connective Tissue Growth Factor - metabolism
Gastric epithelial cells
Gastrin
Gastrins - metabolism
Humans
Invasion
Mice
Migration
Neoplasm Invasiveness - pathology
Signal Transduction
Stomach - metabolism
Stomach - pathology
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Connective tissue growth factor (CTGF) has been reported in gastric adenocarcinoma and in carcinoid tumors. The aim of this study was to explore a possible link between CTGF and gastrin in gastric epithelial cells and to study the role of CTGF in gastrin induced migration and invasion of AGS-GR cells. The effects of gastrin were studied using RT-qPCR, Western blot and assays for migration and invasion. We report an association between serum gastrin concentrations and CTGF abundancy in the gastric corpus mucosa of hypergastrinemic subjects and mice. We found a higher expression of CTGF in gastric mucosa tissue adjacent to tumor compared to normal control tissue. We showed that gastrin induced expression of CTGF in gastric epithelial AGS-GR cells via MEK, PKC and PKB/AKT pathways. CTGF inhibited gastrin induced migration and invasion of AGS-GR cells. We conclude that CTGF expression is stimulated by gastrin and involved in remodeling of the gastric epithelium. •CTGF is overexpressed in gastric cancer and adjacent tissue compared to normal gastric tissue.•Gastrin induces expression of CTGF in gastric epithelial cells.•CTGF inhibits gastrin induced migration and invasion of AGS-GR cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2016.05.052