An improved chromosome preparation from male gametophyte of Laminaria japonica (Heterokontophyta)

Issue Title: Asian Pacific Phycology in the 21st Century: Prospects and Challenges An improved method for the preparation of chromosomes from the male gametophyte of the alga Laminaria japonica Aresch. was described. The male gametophyte was pretreated with pDB (p-dichlorobenzene) and 8-hydroxyquino...

Full description

Saved in:
Bibliographic Details
Published in:Hydrobiologia 2004-01, Vol.512 (1-3), p.141-144
Main Authors: Ran Zhou, Li, Xun Dai, Ji, Dong Shen, Song
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Algae
Biological and medical sciences
Chromosomes
Dichlorobenzene
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Genetics
Laminaria japonica
Phycology
Plant aquaculture, algal production (macroalgae)
Plant reproduction
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Issue Title: Asian Pacific Phycology in the 21st Century: Prospects and Challenges An improved method for the preparation of chromosomes from the male gametophyte of the alga Laminaria japonica Aresch. was described. The male gametophyte was pretreated with pDB (p-dichlorobenzene) and 8-hydroxyquinoline in order to clear cell wall and soften cytoplasm. The samples were treated by mordant iron alum [FeNH^sub 4^ (SO^sub 4^)^sub 2^6H^sub 2^O] followed by staining with haemotoxylin. Well-spread and highly stained chromosomes were observed without precipitation. The chromosome number of male gametophyte of L. japonica was estimated to be 31. %[PUBLICATION ABSTRACT]
ISSN:0018-8158
1573-5117
DOI:10.1023/B:HYDR.0000020319.36532.eb