Development of meniscus substitutes using a mixture of biocompatible polymers and extra cellular matrix components by electrospinning

Despite the significant advances in the meniscus tissue engineering field, it is difficult to recreate the complex structure and organization of the collagenous matrix of the meniscus. In this work, we developed a meniscus prototype to be used as substitute or scaffold for the regeneration of the me...

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Bibliographic Details
Published in:Materials Science & Engineering C 2016-04, Vol.61, p.893-905
Main Authors: López-Calzada, G., Hernandez-Martínez, A.R., Cruz-Soto, M., Ramírez-Cardona, M., Rangel, D., Molina, G.A., Luna-Barcenas, G., Estevez, M.
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Biocompatible Materials - chemistry
Biological implants
Cellular
Collagen
Collagen - chemistry
Collagens
Compressive Strength
Elastic Modulus
Electrospinning
Female
Kidney - pathology
Lactic Acid - chemistry
Menisci, Tibial - physiology
Meniscus
Microscopy, Electron, Scanning
Molds
Morphology
Polyesters
Polymers
Polymers - chemistry
Rats
Rats, Wistar
Regeneration
Tissue Engineering
Tissue-based implants
X-Ray Diffraction
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Summary:Despite the significant advances in the meniscus tissue engineering field, it is difficult to recreate the complex structure and organization of the collagenous matrix of the meniscus. In this work, we developed a meniscus prototype to be used as substitute or scaffold for the regeneration of the meniscal matrix, recreating the differential morphology of the meniscus by electrospinning. Synthetic biocompatible polymers were combined with the extracellular matrix component, collagen and used to replicate the meniscus. We studied the correlation between mechanical and structural properties of the polymer blend as a function of collagen concentration. Fibers were collected on a surface of a rapidly rotating precast mold, to accurately replicate each sectional morphology of the meniscus; different electro-tissues were produced. Detailed XRD analyses exhibited structural changes developed by electrospinning. We achieved to integrate all these electro-tissues to form a complete synthetic meniscus. Vascularization tests were performed to assess the potential use of our novel polymeric blend for promising meniscus regeneration. [Display omitted] •Meniscus-like scaffolds were produced by electrospinning.•Meniscus-like scaffolds were produced using a simple and innovative procedure.•Vascularization on meniscus-like scaffolds was observed.•XRD analyses exhibited structural changes promoted by electrospinning processing.
ISSN:0928-4931
1873-0191
DOI:10.1016/j.msec.2016.01.018