Rod-shaped and substituted hydroxyapatite nanoparticles stimulating type 1 and 2 cytokine secretion

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2016-03, Vol.139, p.10-16
• Main Authors: Wang, Xiupeng, Li, Xia, Ito, Atsuo, Watanabe, Yohei, Sogo, Yu, Hirose, Motohiro, Ohno, Tadao, Tsuji, Noriko M.
• Format: Article
• Language: English
• Subjects: Adjuvant; Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - pharmacology; Animals; Biomedical materials; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - immunology; Cytokines; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - immunology; Durapatite - chemistry; Durapatite - pharmacology; Femur - cytology; Femur - immunology; Hydroxyapatite; Interferon-gamma - biosynthesis; Interferon-gamma - secretion; Interleukin-4 - biosynthesis; Interleukin-4 - secretion; Magnesium; Magnesium - chemistry; Mice; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Particle Size; Primary Cell Culture; Rod-shaped HA; Secretions; Substitution; Surgical implants; Th1 immunity; Th1-Th2 Balance - drug effects; Th2 immunity; Zinc; Zinc - chemistry
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2015.12.004

[Display omitted] •Rod-shaped HA, MgHA and ZnHA nanoparticles were synthesized.•Rod-shaped HA, MgHA and ZnHA nanoparticles promoted the cellular uptake.•Rod-shaped MgHA and ZnHA nanoparticles promoted both Th1 and Th2 cytokine secretion. A Th1 immune response is required for modern vaccines as the most commonly used alum adjuvant has weak capacity for inducing Th1 immune response. Herein, rod-shaped hydroxyapatite (HA), magnesium-substituted HA (MgHA) and zinc-substituted HA (ZnHA) nanoparticles with irregular nanopores were synthesized and used as immunoadjuvants. Magnesium and zinc substitution in HA showed no influence on morphology, particle size, zeta potential and surface area of the nanoparticles. The rod-shaped MgHA and ZnHA nanoparticles promoted the cellular uptake of a molecular immunopotentiator, stimulated both type 1 and 2 cytokine secretion in vitro that relate to Th1 and Th2 immunity of bone marrow dentritic cells, respectively. The MgHA and ZnHA nanoparticles may be useful as immunoadjuvants for human.