Protection against Amorimia septentrionalis poisoning in goats by the continuous administration of sodium monofluoroacetate-degrading bacteria

• Published in: Toxicon (Oxford) 2016-03, Vol.111, p.65-68
• Main Authors: da Silva, Layze C.A., Pessoa, Danielle A.N., Lopes, Jose R.G., de Albuquerque, Laio G., da Silva, Leomyr S.A., Junior, Felicio Garino, Riet-Correa, Franklin
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacteria - classification; Bacteria - metabolism; Burkholderia; Burkholderia sp; Consumption; Cupriavidus sp; Female; Fluoroacetates - chemistry; Fluoroacetates - metabolism; Goat Diseases - chemically induced; Goat Diseases - microbiology; Goat Diseases - prevention & control; Goats; Inoculation; Male; Malpighiaceae - chemistry; Malpighiaceae - toxicity; Paenibacillus; Paenibacillus sp; Plant Poisoning - prevention & control; Plant Poisoning - veterinary; Plants, Toxic - chemistry; Plants, Toxic - toxicity; Poisoning; Ralstonia; Ralstonia sp; Sodium; Sodium monofluoroacetate; Sudden death
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2015.12.016

The intraruminal inoculation of sodium monofluoroacetate (MFA)-degrading bacteria has been proposed as a method to prevent poisoning by MFA-containing plants. In previous experiments, MFA-degrading bacteria were inoculated intraruminally before or concurrent with plant challenge, with both strategies conferring partial protection to poisoning. To evaluate the protection to Amorimia septentrionalis poisoning provided by the continuous inoculation of MFA-degrading bacteria isolated from plants and soils, 18 goats were divided into three experimental groups of six animals each: Group 1 goats received daily doses of a mixture of Paenibacillus sp. and Cupriavidus sp., and Group 2 goats received a mixture of Ralstonia sp. and Burkholderia sp., for 40 days, while Group 3 goats were not inoculated. Ten days after initiation of bacterial inoculation in Groups 1 and 2, all goats were challenged daily with 5 g/kg body weight of green leaves from A. septentrionalis. Four goats from Group 1 consumed the leaves throughout the 30-day consumption period and showed clinical signs such as transient tachycardia and engorgement of the jugular. The two remaining animals from Group 1 showed obvious signs of intoxication, and plant administration was suspended on days 17 and 19. The goats in Group 2 consumed the leaves throughout the 30-day study without showing signs of poisoning. The goats from Group 3 (control) manifested severe clinical signs of poisoning between the 3rd and 10th days following the start of the A. septentrionalis challenge. Under the conditions of this experiment continuous intraruminal administration of Ralstonia sp. and Burkholderia sp. provided complete protection to poisoning by A. septentrionalis in goats, while continuous intraruminal administration of Paenibacillus sp. and Cupriavidus sp. provided partial protection. •Amorimia septentrionalis is a toxic plant containing sodium monofluoroacetate (MFA).•In the rumen, MFA-degrading bacteria confer partial protection to the poisoning.•Ralstonia sp. and Burkholderia sp. are environmental MFA-degrading bacteria.•Continuous administration of these bacteria conferred protection to this plant poisoning.•Probiotics of these bacteria may be used to avoid MFA-containing plant poisonings.