Sampling frequency, response times and embedded signal filtration in fast, high efficiency liquid chromatography: A tutorial

With increasingly efficient columns, eluite peaks are increasingly narrower. To take full advantage of this, choice of the detector response time and the data acquisition rate a.k.a. detector sampling frequency, have become increasingly important. In this work, we revisit the concept of data samplin...

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Bibliographic Details
Published in:Analytica chimica acta 2016-02, Vol.907, p.31-44
Main Authors: Wahab, M. Farooq, Dasgupta, Purnendu K., Kadjo, Akinde F., Armstrong, Daniel W.
Format: Article
Language:English
Subjects:
Detectors
Digital filters
Fast separations
Filtering
Filtration
Focusing
High-efficiency chromatography
Liquid chromatography
Noise
Response time
Response times
Sampling
Sampling theorem
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Summary:With increasingly efficient columns, eluite peaks are increasingly narrower. To take full advantage of this, choice of the detector response time and the data acquisition rate a.k.a. detector sampling frequency, have become increasingly important. In this work, we revisit the concept of data sampling from the theorem variously attributed to Whittaker, Nyquist, Kotelnikov, and Shannon. Focusing on time scales relevant to the current practice of high performance liquid chromatography (HPLC) and optical absorbance detection (the most commonly used method), even for very narrow simulated peaks Fourier transformation shows that theoretical minimum sampling frequency is still relatively low (
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2015.11.043