Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement

Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co­(II) complex of a cyanine dye, which shows...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-02, Vol.88 (3), p.1526-1529
Main Authors: Oshikawa, Yuji, Furuta, Kazuyuki, Tanaka, Satoshi, Ojida, Akio
Format: Article
Language:English
Subjects:
Cell Degranulation
Cell Line
Cells
Cobalt - chemistry
Displacement
Dyes
Fluorescence
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
Histamine - analysis
Histamine - metabolism
Histamines
Humans
Imaging
Mast Cells - cytology
Mast Cells - physiology
Masts
Molecular Structure
Organic chemicals
Organometallic Compounds - chemistry
Real time
Single-Cell Analysis - methods
Stimuli
Time Factors
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Summary:Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co­(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b04758