High-density antimicrobial peptide coating with broad activity and low cytotoxicity against human cells

• Published in: Acta biomaterialia 2016-03, Vol.33, p.64-77
• Main Authors: Rai, Akhilesh, Pinto, Sandra, Evangelista, Marta B., Gil, Helena, Kallip, Silvar, Ferreira, Mario G.S., Ferreira, Lino
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - pharmacology; Antiinfectives and antibacterials; Antimicrobial peptide; Antimicrobial properties; Au NPs-coated surface; Bacteria; Biocompatibility; Cecropin melittin; Cecropins - pharmacology; Cell Death - drug effects; Cell Membrane - drug effects; Cell Membrane - metabolism; Coated Materials, Biocompatible - pharmacology; Coating; Escherichia coli; Fibroblasts - cytology; Fibroblasts - drug effects; Gold - pharmacology; Human; Human Umbilical Vein Endothelial Cells - cytology; Human Umbilical Vein Endothelial Cells - drug effects; Humans; Immobilized Proteins - pharmacology; Medical devices; Microbial Sensitivity Tests; Microorganisms; Nanoparticles; Peptides; Peptides - pharmacology; Photoelectron Spectroscopy; Serum - metabolism; Surface chemistry; Surface Properties
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2016.01.035

[Display omitted] Medical device-associated infections are a multi-billion dollar burden for the worldwide healthcare systems. The modification of medical devices with non-leaching coatings capable of killing microorganisms on contact is one of the strategies being investigated to prevent microorganism colonization. Here we developed a robust antimicrobial coating based on the chemical immobilization of the antimicrobial peptide (AMP), cecropin-melittin (CM), on gold nanoparticles coated surfaces. The concentration of AMP immobilized (110μg/cm2) was higher than most of the studies reported so far (