Urease-based ISFET biosensor for arginine determination

In this work a novel biosensor for arginine determination based on the urease inhibition effect has been proposed. Ion-selective field effect transistors were used as transducers. Urease immobilized in glutaraldehyde vapor served as a biorecognition element of the biosensor. Significant part of the...

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Bibliographic Details
Published in:Talanta (Oxford) 2014-04, Vol.121, p.18-23
Main Authors: Sheliakina, M., Arkhypova, V., Soldatkin, O., Saiapina, O., Akata, B., Dzyadevych, S.
Format: Article
Language:English
Subjects:
Amino acids
Animals
Arginine
Arginine - analysis
Biosensing Techniques
Biosensor
Biosensors
Cattle
Glutaraldehyde
Inhibition
Ion-selective field effect transistors
Limit of Detection
Optimization
Reproducibility
Transducers
Ureas
Urease
Urease - chemistry
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Summary:In this work a novel biosensor for arginine determination based on the urease inhibition effect has been proposed. Ion-selective field effect transistors were used as transducers. Urease immobilized in glutaraldehyde vapor served as a biorecognition element of the biosensor. Significant part of the work was aimed at proving the urease inhibition by arginine. Optimal concentration of urea for arginine determination was chosen. Detection limit for arginine was 0.05mM. The biosensor selectivity towards different amino acids was studied. The results of quantitative determination of l-arginine in the real sample (a drinkable solution “Arginine Veyron”) were in good agreement with the producer's data (a relative error was 5.2%). The biosensor showed a good reproducibility of arginine determination. [Display omitted] •A possibility of arginine determination by urease-ISFET biosensor has been shown.•Indirect competitive inhibition of urease by arginine has been demonstrated.•Different amino acids did not cause inhibitory effect on urease, except lysine and cistine.•Proposed biosensor can be further used for arginine determination in real solutions.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2013.12.042