A droplet-based microfluidic immunosensor for high efficiency melamine analysis

We report a droplet-based microfluidic immunosensor for the rapid and accurate detection of melamine, an organic base that has been implicated in widescale adulteration of food products such as milk. Our melamine assay is based on the competitive reaction between native melamine and a melamine-fluor...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2016-06, Vol.80, p.182-186
Main Authors: Choi, Jae-Won, Min, Kyong-Mi, Hengoju, Sundar, Kim, Gil-Jung, Chang, Soo-Ik, deMello, Andrew J., Choo, Jaebum, Kim, Hak Yong
Format: Article
Language:English
Subjects:
Animals
Assaying
Biosensing Techniques
Biotechnology
Conjugates
Droplet-based microfluidics
ELISA
Enzyme-Linked Immunosorbent Assay
Fluorescence polarization
Food Analysis
Foods
Humans
Immunosensors
Melamine
Microfluidics
Milk
Milk - chemistry
Triazines - chemistry
Triazines - isolation & purification
United States
United States Food and Drug Administration
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Summary:We report a droplet-based microfluidic immunosensor for the rapid and accurate detection of melamine, an organic base that has been implicated in widescale adulteration of food products such as milk. Our melamine assay is based on the competitive reaction between native melamine and a melamine-fluorescein isothiocyanate (FITC) conjugate against an anti-hapten antibody. The adoption of fluorescence polarization, allows the quantification of melamine in a more direct and rapid manner than established heterogeneous methods based on liquid chromatography, mass spectrometry, and enzyme-linked immunosorbent assay (ELISA). The detection protocol provides a limit of detection of 300ppb, which is below the maximum allowable melamine levels (2.5ppm) defined by the U.S. Food and Drug Administration and the European Commission to a significant extent. •We report a droplet-based microfluidic immunosensor for the detection of melamine.•Fluorescence polarization-based microdroplet sensor platform has been developed.•This approach provides a cost-effective detection of melamine in milk.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2015.12.023