SiO sub(2)-based nanobiosensor monitoring toxicological behavior of Mitoxantrone in vitro

The present study involves the development of nanobiosensor to determine toxicological behavior of Mitoxantrone (MTX). Mitoxantrone intercalates with DNA and produces MTX-DNA adduct, resulting in blockade of protein synthesis and excessive production of free radicals in the myocardium eventually lea...

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Bibliographic Details
Published in:Applied nanoscience 2014-06, Vol.4 (5), p.523-529
Main Authors: Lad, Amitkumar N, Agrawal, Y K
Format: Article
Language:English
Subjects:
Adducts
Deoxyribonucleic acid
Electrodes
Free radicals
Monitoring
Nanostructure
Silicon dioxide
Toxicity
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Summary:The present study involves the development of nanobiosensor to determine toxicological behavior of Mitoxantrone (MTX). Mitoxantrone intercalates with DNA and produces MTX-DNA adduct, resulting in blockade of protein synthesis and excessive production of free radicals in the myocardium eventually leads to cardiac toxicity. Potentiometry was applied to develop an electroanalytical procedure for the determination of MTX and its interaction with DNA immobilized on the electrode surface modified with Silicon dioxide (SiO sub(2)) nanoparticles. The nanobiosensor immersed in MTX solution to monitor MTX-DNA interaction with respect to time and alters the resistance of the nanobiosensor. It was observed that MTX-DNA interaction is fast initially and as time elapses, the change in interaction gets slow due to formation of MTX-DNA adduct. Determination limit of the nanobiosensor is 100-10 ng/ml. This study suggests that the nanobiosensor allows real-time monitoring of the drug-DNA interaction changes by measuring the potential at sensor interface which can prove to be an important tool in drug discovery pipelines and molecular toxicology.
ISSN:2190-5509
2190-5517
DOI:10.1007/s13204-013-0239-4