MONITORING OF GLUCOSE CONSUMPTION AND LACTATE PRODUCTION BY ENDOTHELIAL CELLS CULTURED IN CAPILLARY MEMBRANE BIOREACTORS

Aim: The objective is to monitor glucose consumption and lactate production by human umbilical vein endothelial cells (HUVEC) cultured in capillary membrane bioreactors to be used as a model of a blood vessel. Methods: Isolated HUVECs were cultured in modified Medium 199. After the second passage, H...

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Bibliographic Details
Published in:International journal of artificial organs 2014-01, Vol.37 (8), p.595-595
Main Authors: Ciechanowska, A, Ladyzynski, P, Hoser, G, Sabalinska, S, Kawiak, J, Chwojnowski, A, Wojciechowski, C, Wojcicki, J M
Format: Article
Language:English
Subjects:
Bioreactors
Capillarity
Consumption
Culture
Density
Endothelial cells
Glucose
Lactates
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Summary:Aim: The objective is to monitor glucose consumption and lactate production by human umbilical vein endothelial cells (HUVEC) cultured in capillary membrane bioreactors to be used as a model of a blood vessel. Methods: Isolated HUVECs were cultured in modified Medium 199. After the second passage, HUVECs were introduced into bioreactors in different quantities (2, 4, 6 and 15 x 10 super(4) cells per cm super(2)). Bioreactors were rotated for 4 h to seed cells on the inner surface of capillaries. We used original bioreactors with 14 polysulfone capillaries, inner diameter of 640 mu m and total surface area of 16.3 cm super(2) covered with fibronectin to promote cells attachment. HUVECs were subjected to the shear stress resulting from medium flow generated by a peristaltic pump. Glucose and lactate were measured every 24 h in the circulating medium for 4 days. Results: Glucose consumption was the highest during the first day of culture and then it stayed relatively stable. Lactate production was stable during the whole monitored period in all cultures but the one with 150,000 cells per cm super(2). In this case, cells produced 3.5 times more lactate during the first day than in the other cultures, which might be attributed to a limited oxygen supply for such an amount of cells. Conclusions: The obtained results suggest that HUVECs can be safely seeded in the assessed bioreactors with densities ranging from 2 to 6 x 10 super(4) cells/cm super(2) and that cell cultures stabilize after 24 to 48 h.
ISSN:0391-3988
DOI:10.5301/ijao.5000346