A Rapid and Sensitive UPLC–MS/MS Method for Determination of Docetaxel in Rabbit Plasma: Pharmacokinetic Study of New Lung-Targeting Docetaxel Liposome at Low Dose

In this study, a more rapid and more sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS) method is developed and validated for the pharmacokinetic study of a new lung-targeting docetaxel liposome (DTX-LP) at low dose (1 mg/kg) in rabbits. The method is reliable an...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2015-12, Vol.73 (3), p.623-629
Main Authors: Wang, Jie, Lan, Zuoping, Zhang, Li, Guo, Hongmei, Liu, Zhonghong, Yu, Yu
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - administration & dosage
Antineoplastic Agents - blood
Antineoplastic Agents - pharmacokinetics
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Cell Biology
Chromatography, Liquid - methods
Intravenous administration
Life Sciences
Liposomes - chemistry
Liquid chromatography
Lung - metabolism
Mass spectrometry
Mass Spectrometry - methods
Original Paper
Pharmacokinetics
Pharmacology/Toxicology
Rabbits
Sensitivity and Specificity
Taxoids - administration & dosage
Taxoids - blood
Taxoids - pharmacokinetics
Tissue Distribution
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Summary:In this study, a more rapid and more sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS) method is developed and validated for the pharmacokinetic study of a new lung-targeting docetaxel liposome (DTX-LP) at low dose (1 mg/kg) in rabbits. The method is reliable and reproducible with intra-day precision below 5.75 %, inter-day precision below 7.57 %, and mean extraction recovery of 84.1–91.7 %. At low dose, the validated method is successfully applied to the comparative pharmacokinetic study of docetaxel (DTX) in rabbit plasma after intravenous administration of DTX-LP and DTX injection, respectively. The results indicate that the pharmacokinetic profile of DTX is completely changed when loaded in the new type of liposome, which can make DTX quicker distribution from the circulation to the target organ and slower eliminated from the body.
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-015-0639-z