Quantitative Proteomics Reveals the Flooding-Tolerance Mechanism in Mutant and Abscisic Acid-Treated Soybean

Flooding negatively affects the growth of soybean, and several flooding-specific stress responses have been identified; however, the mechanisms underlying flooding tolerance in soybean remain unclear. To explore the initial flooding tolerance mechanisms in soybean, flooding-tolerant mutant and absci...

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Bibliographic Details
Published in:Journal of proteome research 2016-06, Vol.15 (6), p.2008-2025
Main Authors: Yin, Xiaojian, Nishimura, Minoru, Hajika, Makita, Komatsu, Setsuko
Format: Article
Language:English
Subjects:
Abscisic Acid - pharmacology
Adaptation, Physiological - drug effects
Floods
Gene Expression Regulation, Plant
Glycine max - chemistry
Glycine max - physiology
Mutation
Plant Proteins - analysis
Plant Roots
Protein Biosynthesis
Proteomics - methods
Stress, Physiological
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Summary:Flooding negatively affects the growth of soybean, and several flooding-specific stress responses have been identified; however, the mechanisms underlying flooding tolerance in soybean remain unclear. To explore the initial flooding tolerance mechanisms in soybean, flooding-tolerant mutant and abscisic acid (ABA)-treated plants were analyzed. In the mutant and ABA-treated soybeans, 146 proteins were commonly changed at the initial flooding stress. Among the identified proteins, protein synthesis-related proteins, including nascent polypeptide-associated complex and chaperonin 20, and RNA regulation-related proteins were increased in abundance both at protein and mRNA expression. However, these proteins identified at the initial flooding stress were not significantly changed during survival stages under continuous flooding. Cluster analysis indicated that glycolysis- and cell wall-related proteins, such as enolase and polygalacturonase inhibiting protein, were increased in abundance during survival stages. Furthermore, lignification of root tissue was improved even under flooding stress. Taken together, these results suggest that protein synthesis- and RNA regulation-related proteins play a key role in triggering tolerance to the initial flooding stress in soybean. Furthermore, the integrity of cell wall and balance of glycolysis might be important factors for promoting tolerance of soybean root to flooding stress during survival stages.
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.6b00196