Two Oyster Species That Show Differential Susceptibility to Virus Infection Also Show Differential Proteomic Responses to Generic dsRNA

Viral diseases are a significant cause of mortality and morbidity in oysters, resulting in significant economic losses. We investigated the proteomic responses of these two species of oysters to generic double-stranded RNAs (poly I:C and poly A:U). Analysis of proteomic data using isobaric tags for...

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Bibliographic Details
Published in:Journal of proteome research 2016-06, Vol.15 (6), p.1735-1746
Main Authors: Masood, Muhammad, Raftos, David A, Nair, Sham V
Format: Article
Language:English
Subjects:
Animals
Disease Susceptibility
Gene Ontology
Ostreidae - virology
Poly A-U - pharmacology
Poly I-C - pharmacology
Proteome - drug effects
Proteomics - methods
RNA, Double-Stranded - pharmacology
Transcriptome
Virus Diseases - pathology
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Summary:Viral diseases are a significant cause of mortality and morbidity in oysters, resulting in significant economic losses. We investigated the proteomic responses of these two species of oysters to generic double-stranded RNAs (poly I:C and poly A:U). Analysis of proteomic data using isobaric tags for relative and absolute quantitaion (iTRAQ) indicated that there were significant differences in the proteomic responses of the two oyster species resulting from this treatment. Gene ontology analysis showed that several biological processes, cellular components, and molecular function were unique to the different data sets. For example, a number of proteins implicated in the TLR signaling pathway were associated with the Saccostrea glomerata data set but were absent in the Crassostra gigas data set. These results suggest that the differences in the proteomic responses to dsRNA may underpin the biological differences in viral susceptibility. Molecular targets previously shown to be expressed in C. gigas in response to OsHV1 infections were not present in our proteomic data sets, although they were present in the RNA extracted from the very same tissues. Taken together, our data indicate that there are substantial disparities between transcriptomic and proteomic responses to dsRNA challenge, and a comprehensive account of the oysters’ biological responses to these treatments must take into account that disparity.
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.5b00615