Isolation and chemical evaluation of protein from shrimp cannery effluent

• Published in: Journal of agricultural and food chemistry 1975-07, Vol.23 (4), p.632-635
• Main Authors: Toma, Ramses B, Meyers, Samuel P
• Format: Article
• Language: English
• Subjects: Amino Acids - analysis; Decapoda (Crustacea); Food-Processing Industry; Freshwater; Industrial Waste; Penaeus; Proteins - analysis; Proteins - isolation & purification; Shellfish; Waste Disposal, Fluid
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf60200a012

Shrimp cannery effluent, collected at a seafood plant at Westego, La., contained {approx} 0.65% total solids. Approx 2 kg of shrimp waste protein (SWP), drum dried at 124-127{degree}, was obtained from 2650 l of the effluent by treatment with HCl at the isoelectric point. Proximate analyses of SWP were: moisture, 10.00; ash, 6.33; protein, 58.98; fat, 16.97; and crude fiber, 1.62%. Gross energy was 5170 cal/g; microminerals were: Ca, 0.465%; Mg, 471 ppm; p, 0.815%; S, 0.415%; Fe, 0.110%; Zn, 109.5 ppm; Mn, 18 ppm; and Cu, 17.5 ppm. Total bacterial counts for shrimp cannery effluent and SWP were 1.4 x 10 Super(6)/ml and < 200/g, respectively. SWP was analyzed for 18 amino acids. Hygroscopic properties of SWP and its defatted derivative were determined by exposure to atmospheric humidity and to 100% relative humidity in a closed container. Also, preliminary clarification tests were made with dilute solutions of 4 inorganic salts, namely aluminium sulfate, ferric sulfate ferric chloride, and sodium silicate. The 2 Fe salts were the most effective in separation.