Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuant® system

Highlights • The PowerQuant® System was developed to quantify human DNA and assess sample quality. • A developmental validation following SWGDAM guidelines was completed. • Degraded DNA, inhibited samples, and mixtures of male/female DNA were evaluated. • Results demonstrate reliability, reproducibi...

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Bibliographic Details
Published in:Forensic science international : genetics 2016-07, Vol.23, p.166-177
Main Authors: Ewing, Margaret M, Thompson, Jonelle M, McLaren, Robert S, Purpero, Vincent M, Thomas, Kelli J, Dobrowski, Patricia A, DeGroot, Gretchen A, Romsos, Erica L, Storts, Douglas R
Format: Article
Language:English
Subjects:
Animals
Chromosomes, Human, Y
Continental Population Groups - genetics
DNA - analysis
DNA Degradation, Necrotic
DNA Fingerprinting
Humans
Male
Microsatellite Repeats
Pathology
Polymerase Chain Reaction - instrumentation
Reproducibility of Results
Sensitivity and Specificity
Species Specificity
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Summary:Highlights • The PowerQuant® System was developed to quantify human DNA and assess sample quality. • A developmental validation following SWGDAM guidelines was completed. • Degraded DNA, inhibited samples, and mixtures of male/female DNA were evaluated. • Results demonstrate reliability, reproducibility and suitability for forensic use. • The PowerQuant® System distinguishes degraded samples from inhibited samples.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2016.04.007