D5S2500 is an ambiguously characterized STR: Identification and description of forensic microsatellites in the genomics age

Highlights • An STR named D5S2500 is part of two established forensic multiplexes: Qiagen’s HDplex and AGCU ScienTech’s non-CODIS STR 21plex. • The AGCU D5S2500 marker, originally developed as a miniSTR, does not match the details of D5S2500 in NCBI’s Probe microsatellite database. • Genomic charact...

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Bibliographic Details
Published in:Forensic science international : genetics 2016-07, Vol.23, p.19-24
Main Authors: Phillips, C, Parson, W, Amigo, J, King, J.L, Coble, M.D, Steffen, C.R, Vallone, P.M, Gettings, K.B, Butler, J.M, Budowle, B
Format: Article
Language:English
Subjects:
AGCU 21plex
D5S2500
DNA Fingerprinting
Electrophoresis, Capillary
Gene Frequency
Genetic Markers
Humans
Microsatellite Repeats
miniSTRs
Multiplex Polymerase Chain Reaction
Non CODIS STRs
Pathology
Qiagen HDplex
Short tandem repeats
STRs
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Description
Summary:Highlights • An STR named D5S2500 is part of two established forensic multiplexes: Qiagen’s HDplex and AGCU ScienTech’s non-CODIS STR 21plex. • The AGCU D5S2500 marker, originally developed as a miniSTR, does not match the details of D5S2500 in NCBI’s Probe microsatellite database. • Genomic characteristics are outlined for both loci, confirming the HDplex D5S2500 STR is correctly identified. • To avoid future ambiguities, a recommended minimum genomic data framework for novel forensic markers is proposed, plus the name D5S2800 for the component STR of the AGCU 21plex.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2016.03.002