Evaluation of the RapidHIT™ 200 and RapidHIT GlobalFiler® Express kit for fully automated STR genotyping

Highlights • The RapidHIT 200/GlobalFiler Express Rapid DNA system was evaluated as an expert system for genotyping buccal reference samples. • STR profiles exhibited artifacts and low peak intensities that required manual data review. • With manual data review, the success rate for obtaining a full...

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Bibliographic Details
Published in:Forensic science international : genetics 2016-07, Vol.23, p.1-8
Main Authors: Date-Chong, Mavis, Hudlowa, William R, Buoncristiani, Martin R
Format: Article
Language:English
Subjects:
DNA Fingerprinting - instrumentation
Electrophoresis, Capillary
Genetic Markers
Genotype
GlobalFiler® Express
Humans
Microsatellite Repeats
Modified Rapid DNA analysis
Mouth Mucosa - cytology
Pathology
Rapid DNA
RapidHIT™ 200
Reproducibility of Results
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Summary:Highlights • The RapidHIT 200/GlobalFiler Express Rapid DNA system was evaluated as an expert system for genotyping buccal reference samples. • STR profiles exhibited artifacts and low peak intensities that required manual data review. • With manual data review, the success rate for obtaining a full genotype at each locus in the STR kit was ∼91%. • With expert-only (no manual) review, the corresponding success rate was ∼50%.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2016.03.001