Surface binding, localization and storage of iron in the giant kelp Macrocystis pyrifera

Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. Although the iron uptake and storage mechanisms of terrestrial/higher plants have been well-studied, the corresponding systems in...

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Bibliographic Details
Published in:Metallomics 2016-04, Vol.8 (4), p.403-411
Main Authors: Miller, Eric P, Auerbach, Hendrik, Schünemann, Volker, Tymon, Teresa, Carrano, Carl J
Format: Article
Language:English
Subjects:
3,3'-Diaminobenzidine - metabolism
Azoles - metabolism
Cell Membrane - metabolism
Ectocarpus
Fluorescence
Intracellular Space - metabolism
Iron - metabolism
Kinetics
Macrocystis
Macrocystis - metabolism
Macrocystis pyrifera
Spectroscopy, Mossbauer
Thermodynamics
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Summary:Iron is an essential element for all living organisms due to its ubiquitous role in redox and other enzymes, especially in the context of respiration and photosynthesis. Although the iron uptake and storage mechanisms of terrestrial/higher plants have been well-studied, the corresponding systems in marine algae have received far less attention. While the iron many marine algae take up from the environment, irrespective of its detailed internalization mechanism, arrives at the cell surface by diffusion, there is growing evidence for more "active" means of concentrating this element prior to uptake. It has been well established in both laboratory and environmentally derived samples, that a large amount of iron can be "non-specifically" adsorbed to the surface of marine algae. While this phenomenon is widely recognized and has prompted the development of experimental protocols to eliminate its contribution to iron uptake studies, its potential biological significance as a concentrated iron storage source for marine algae is only now being recognized. In this study, using an interdisciplinary array of techniques, we show that the giant kelp Macrocystis pyrifera also displays significant cell surface bound iron although less than that seen with the related brown alga Ectocarpus siliculosus. The iron on the surface is likely bound to carboxylate groups and once inside the iron is found to localize differently depending on cell type. Iron appears to be stored in an as yet undefined mineral phase.
ISSN:1756-5901
1756-591X
DOI:10.1039/c6mt00027d