Blue 'turn-on' fluorescent probes for the direct detection of free radicals and nitric oxide in Pseudomonas aeruginosabiofilms

Two novel cell-permeable and bacteria-compatible 'turn-on' fluorescent probes, designed to be compatible in a multi-dye system and to fluoresce in the blue region exhibiting emission maxima of 440-490 nm, were synthesized. The profluorescent nitroxide probe (11) was developed to visualize...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2016-02, Vol.14 (7), p.2272-2281
Main Authors: Barzegar Amiri Olia, Mina, Zavras, Athanasios, Schiesser, Carl H, Alexander, Stefanie-Ann
Format: Article
Language:English
Subjects:
Pseudomonas
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Summary:Two novel cell-permeable and bacteria-compatible 'turn-on' fluorescent probes, designed to be compatible in a multi-dye system and to fluoresce in the blue region exhibiting emission maxima of 440-490 nm, were synthesized. The profluorescent nitroxide probe (11) was developed to visualize and quantify free radical and redox processes, and the Cu(ii)-complexed coumarin-based probe (16a) was developed for NO detection. Confocal laser-scanning microscopy and subsequent digital analysis of Pseudomonas aeruginosabiofilms stained with 11 or 16a determined that free radical and redox processes and NO generation occur predominantly in live cells during normal biofilm growth.
ISSN:1477-0520
1477-0539
DOI:10.1039/c5ob02441b