Identification of proteins in blood nucleoprotein complexes

Nucleoprotein complexes were isolated from blood plasma by affine chromatography using immobilized polyclonal antihistone antibodies. In blood of healthy subjects the prevalence of histone-containing nucleoprotein complexes, including 170–180 bp DNA, were found, whereas in blood of breast cancer pat...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2015-11, Vol.41 (6), p.617-625
Main Authors: Tamkovich, S. N., Serdukov, D. S., Tutanov, O. S., Duzhak, T. G., Laktionov, P. P.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Life Sciences
Organic Chemistry
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Summary:Nucleoprotein complexes were isolated from blood plasma by affine chromatography using immobilized polyclonal antihistone antibodies. In blood of healthy subjects the prevalence of histone-containing nucleoprotein complexes, including 170–180 bp DNA, were found, whereas in blood of breast cancer patients the 170–180 bp DNA and the DNA containing more than 6000 bp were present in equal quantities. Using MALDI-TOF mass spectrometry, the proteins from histone-containing nucleoprotein complexes were identified that provided transport of extracellular nucleic acids into blood stream. It was found that nucleoprotein complexes from blood of breast cancer patients contained tumor-associated proteins, such as LDOC1L, ADP/ATP translocase 3, and Lamellipodin. These data confirm generation of part of circulating extracellular DNA by tumor cells.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162015060163