Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples

A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. •A rapid...

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Bibliographic Details
Published in:Molecular and cellular probes 2016-04, Vol.30 (2), p.122-124
Main Authors: Ren, Hang, Yang, Mingjuan, Zhang, Guoxia, Liu, Shiwei, Wang, Xinhui, Ke, Yuehua, Du, Xinying, Wang, Zhoujia, Huang, Liuyu, Liu, Chao, Chen, Zeliang
Format: Article
Language:English
Subjects:
Blood - microbiology
Brucella
Brucellosis - blood
Brucellosis - diagnosis
DNA Primers - genetics
Humans
Molecular Diagnostic Techniques - methods
Nucleic Acid Amplification Techniques - methods
Point-of-care diagnosis
Recombinase polymerase amplification
Recombinases - metabolism
Sensitivity and Specificity
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Summary:A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis. •A rapid and sensitive Bruce-RPA assay was developed for detection of Brucella.•The assay could detect as few as 3 copies of Brucella per reaction within 20 min.•It represents a candidate point-of-care diagnosis assay for human brucellosis.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2016.02.007