Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells

The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5 mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its v...

Full description

Saved in:
Bibliographic Details
Published in:Mitochondrion 2016-03, Vol.27, p.40-47
Main Authors: Bianchessi, Valentina, Vinci, Maria Cristina, Nigro, Patrizia, Rizzi, Valeria, Farina, Floriana, Capogrossi, Maurizio C, Pompilio, Giulio, Gualdi, Valentina, Lauri, Andrea
Format: Article
Language:English
Subjects:
5-Methylcytosine - analogs & derivatives
Cytosine - analogs & derivatives
Cytosine - analysis
DNA, Intergenic - chemistry
DNA, Mitochondrial - chemistry
Endothelial Cells - chemistry
Humans
Mitochondria - chemistry
Sequence Analysis, DNA - methods
Sulfites - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5 mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5 mC clusters and a biased distribution of 5 mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5 mC in D-loop formation, rather than in mitochondrial gene expression.
ISSN:1567-7249
1872-8278
DOI:10.1016/j.mito.2016.02.004