Synthesis of butyl butyrate in 93 % yield by Thermomyces lanuginosus lipase on waste eggshells

There is a high demand for immobilized enzymes in the industry because it is a green technology, it improves the efficiency of the crude enzyme and it minimizes the step of product recovery. Selection of a suitable carrier for enzyme immobilization is crucial. Eggshells are abundantly available as a...

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Bibliographic Details
Published in:Environmental chemistry letters 2016-06, Vol.14 (2), p.189-194
Main Authors: Salleh, Suhaila, See, Yen Shan, Serri, N. A., Hena, Sufia, Tajarudin, H. A.
Format: Article
Language:English
Subjects:
Adsorption
Analytical Chemistry
Clean technology
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Enzymes
Geochemistry
Organic wastes
Original Paper
Pollution
Thermomyces lanuginosus
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Summary:There is a high demand for immobilized enzymes in the industry because it is a green technology, it improves the efficiency of the crude enzyme and it minimizes the step of product recovery. Selection of a suitable carrier for enzyme immobilization is crucial. Eggshells are abundantly available as an organic waste. Here, lipase from Thermomyces lanuginosus was immobilized on eggshell to produce butyl butyrate. We evaluated the effect of lipase loading, from 0.5 to 3.0 %, size of eggshells, lower than 1, 1–2 and 2–4 mm, and temperature, from 30 to 50 °C. Results show that the optimum conditions calculated by response surface methodology analysis for butyl butyrate synthesis were 40 % w/v of immobilized lipase loading and 0.2 M of substrate concentration at 38 °C during 24 h. The predicted yield was 92.78 %, and the yield obtained during synthesis was 93.48 %.
ISSN:1610-3653
1610-3661
DOI:10.1007/s10311-016-0553-7