Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season

Abstract The 2014–2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2016-05, Vol.85 (1), p.42-46
Main Authors: Overmeire, Yarah, Vanlaere, Elke, Hombrouck, Anneleen, De Beenhouwer, Hans, Simons, Guus, Brink, Antoinette, Van den Abeele, Anne-Marie, Verfaillie, Charlotte, Van Acker, Jos
Format: Article
Language:English
Subjects:
Antigenic drift
Antigenic Variation - immunology
Humans
Infectious Disease
Influenza
Influenza A virus - classification
Influenza A virus - genetics
Influenza A virus - immunology
Influenza, Human - diagnosis
Influenza, Human - epidemiology
Influenza, Human - immunology
Influenza, Human - virology
Internal Medicine
Laboratory
Laboratory surveillance
Molecular Diagnostic Techniques - methods
Molecular Diagnostic Techniques - standards
Molecular methods
Mutation
Phylogeny
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Reproducibility of Results
RNA, Viral
Seasons
Sensitivity and Specificity
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Summary:Abstract The 2014–2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2016.02.004