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The status of Potato leafroll virus in Kenya [Solanum tuberosum L.]

A survey of potato leaf roll disease conducted in major potato growing regions of Kenya (Meru, Kiambu, Nyamira and Kisii districts) revealed the highest incidence in Kisima location, Timau division of Meru district, and an absence of PLRV in leaf-rolled samples from Githongo location of the same dis...

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Published in:Journal of plant pathology 2003-11, Vol.85 (3), p.153-156
Main Authors: Were, H.K. (Kyushu Univ. (Japan). Graduate School of Bioresources and Bioenvironmental Sciences), Narla, R.D, Nderitu, J.H. (Nairobi Univ. (Kenya). Dept. of Crop Science), Weidemann, H.L. (Biologische Bundesanstalt fur Land und Forstwirtschaft, Braunschweig (Germany). Inst. of Biochemistry and Plant Virology)
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Language:English
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Summary:A survey of potato leaf roll disease conducted in major potato growing regions of Kenya (Meru, Kiambu, Nyamira and Kisii districts) revealed the highest incidence in Kisima location, Timau division of Meru district, and an absence of PLRV in leaf-rolled samples from Githongo location of the same district. The lowest incidence was recorded in Kisii district. The virus was detected in the phloem tissues of potato sprouts and leaves by tissue blot immunoassay (TBIA). The method was found to be simple, rapid and sensitive in detecting PLRV in infected potato plants. In Myzus persicae Sulz., the Acquisition Access Period (AAP), Inoculation Access Period (IAP) and the Latent Period of PLRV, isolated from potato variety Pimpernel collected from Tigoni location, Kiambu district, were typical of PLRV. It is suggested that virus control in potato in Kenya can be made more effective by planting virus-free seed-tubers and by early treatment with insecticides. Additionally, volunteer plants and alternative hosts should be rogued and destroyed because they serve as sources of virus for subsequent infections [Un'indagine sulla malattia dell'arricciamento fogliare della patata nelle principali aree di coltivazione del Kenia (province di Meru, Kyambu, Nyamira e Kisii) ha messo in evidenza l'incidenza piu' elevata a Kisima, area di Timau della provincia di Meru, e l'assenza di PLRV in campioni con foglie accartocciate provenienti da Githongo, localita' della stessa provincia. La minore incidenza e' stata riscontrata nella provincia di Kisii. Il virus e' stato individuato nei tessuti floematici dei germogli e delle foglie di patata mediante saggio immunitario tissue blot (TBIA). Si e' riscontrato che il metodo e' semplice, rapido e sensibile per l'individuazione di PLRV nelle piante di patata infette. In Myzus persicae Sulz., il periodo di accesso all'acquisizione (AAP), il periodo di accesso all'inoculazione (IAP) e il periodo di latenza di PLRV, isolato dalla varieta' di patata Pimpernel raccolta nella localita' di Tigoni, provincia di Kiambu, risultavano quelli tipici di PLRV. Il controllo del virus della patata in Kenia puo' essere reso piu' efficace piantando tuberi-seme esenti da virus e mediante trattamenti precoci con insetticidi. Inoltre, le piante spontanee e gli ospiti alternativi dovrebbero essere rimossi e distrutti, in quanto servono come fonti del virus per infezioni successive]
ISSN:1125-4653
2239-7264